{"id":12287,"date":"2023-03-16T09:31:50","date_gmt":"2023-03-16T09:31:50","guid":{"rendered":"https:\/\/vrfp.es\/simuladores\/laboratorio-de-bioquimica\/"},"modified":"2025-06-25T07:05:39","modified_gmt":"2025-06-25T07:05:39","slug":"biochemistry-laboratory","status":"publish","type":"simuladores","link":"https:\/\/vrfp.es\/en\/simulators\/biochemistry-laboratory\/","title":{"rendered":"Biochemistry Laboratory (I)"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\">DESCRIPTION<\/h3>\n\n\n\n<p>The simulator teaches students about laboratory tools, the main processes that are carried out and the protocols to be taken into account to avoid possible risks.<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">OBJECTIVE<\/h3>\n\n\n\n<p>To teach, in a practical and safe way, the protocols to follow during the most common procedures in a Biochemistry laboratory.<\/p>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">ACTIVITIES<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Use of Colilert<\/strong> to calculate the most probable number (MPN) and detect coliform and E. coli bacteria.<\/li>\n<\/ul>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Western Blot protocol<\/strong> for the identification of proteins according to their molecular weight.<\/li>\n<\/ul>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Purification of DNA<\/strong> using magnetic beads as the most effective option for DNA isolation.<\/li>\n<\/ul>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Cell culture process<\/strong> for the maintenance of cells in vitro, preserving their physiological, biochemical and genetic properties to the maximum.<\/li>\n<\/ul>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Cell staining<\/strong>, fundamental techniques for the precise identification and detailed study of microorganisms.\n<ul class=\"wp-block-list\">\n<li><strong>Positive fungal staining: <\/strong>Allows fungal structures to be visualized and identified by staining them directly.<\/li>\n\n\n\n<li><strong>Negative yeast staining: <\/strong>Uses a dye that does not penetrate the cells, creating a dark contrast in the background and leaving the cells lighter for study.<\/li>\n\n\n\n<li><strong>Differential Gram staining: <\/strong>Classifies bacteria as Gram positive or negative based on the composition of their cell wall.<\/li>\n\n\n\n<li><strong>Specific endospore staining:<\/strong> Highlights endospores within bacterial cells so they can be distinguished.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n","protected":false},"featured_media":10914,"template":"","familias-profesionales":[],"class_list":["post-12287","simuladores","type-simuladores","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/vrfp.es\/en\/wp-json\/wp\/v2\/simuladores\/12287","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/vrfp.es\/en\/wp-json\/wp\/v2\/simuladores"}],"about":[{"href":"https:\/\/vrfp.es\/en\/wp-json\/wp\/v2\/types\/simuladores"}],"version-history":[{"count":5,"href":"https:\/\/vrfp.es\/en\/wp-json\/wp\/v2\/simuladores\/12287\/revisions"}],"predecessor-version":[{"id":15833,"href":"https:\/\/vrfp.es\/en\/wp-json\/wp\/v2\/simuladores\/12287\/revisions\/15833"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/vrfp.es\/en\/wp-json\/wp\/v2\/media\/10914"}],"wp:attachment":[{"href":"https:\/\/vrfp.es\/en\/wp-json\/wp\/v2\/media?parent=12287"}],"wp:term":[{"taxonomy":"familias-profesionales","embeddable":true,"href":"https:\/\/vrfp.es\/en\/wp-json\/wp\/v2\/familias-profesionales?post=12287"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}