DESCRIPTION
A simulator for learning the entire process of DNA extraction and amplification. It allows users to practice preparing materials and reagents, handling bacterial samples, analysing concentration and purity, as well as designing and performing a PCR in a safe and controlled virtual environment.
OBJECTIVE
To allow students to carry out basic molecular biology procedures with technical rigour, understanding each stage and applying the relevant safety measures.
ACTIVITIES
- DNA extraction
- Preparing the necessary equipment for the extraction.
- Collection and handling of bacterial samples.
- Boiling process, adjusting the temperature and timing of the boiling equipment.
- DNA analysis by measuring its concentration and purity.
- Conducting a PCR test:
- Preparation of the reagents required for a PCR reaction.
- PCR design through primer selection and parameter configuration.
- Preparing the PCR mixture in reaction tubes.
- PCR cycling in a thermal cycler, with visual representations of the different stages.
