DESCRIPTION
The simulator teaches students about laboratory tools, the main processes that are carried out and the protocols to be taken into account to avoid possible risks.
OBJECTIVE
To teach, in a practical and safe way, the protocols to follow during the most common procedures in a Biochemistry laboratory.
ACTIVITIES
- Use of Colilert to calculate the most probable number (MPN) and detect coliform and E. coli bacteria.
- Western Blot protocol for the identification of proteins according to their molecular weight.
- Purification of DNA using magnetic beads as the most effective option for DNA isolation.
- Cell culture process for the maintenance of cells in vitro, preserving their physiological, biochemical and genetic properties to the maximum.
- Cell staining, fundamental techniques for the precise identification and detailed study of microorganisms.
- Positive fungal staining: Allows fungal structures to be visualized and identified by staining them directly.
- Negative yeast staining: Uses a dye that does not penetrate the cells, creating a dark contrast in the background and leaving the cells lighter for study.
- Differential Gram staining: Classifies bacteria as Gram positive or negative based on the composition of their cell wall.
- Specific endospore staining: Highlights endospores within bacterial cells so they can be distinguished.
